Last Minute Lecture

Recombinant DNA Technology and Genomics — Gene Cloning, Sequencing, and Functional Genomics Explained | Chapter 16 of Karp’s Cell and Molecular Biology Chapter 16 of Karp’s Cell and Molecular Biology: Concepts and Experiments examines the molecular tools that revolutionized biology, enabling scientists to manipulate DNA, sequence genomes, and investigate gene function with unprecedented precision. The chapter covers foundational methods of recombinant DNA technology, modern genomic approaches, and the transformative applications that arise from these techniques. This expanded summary builds on the video explanation and provides a broad, integrated understanding of how gene manipulation drives discovery and innovation across biotechnology and medicine. For a guided walkthrough of cloning strategies, sequencing technologies, and genomic tools, be sure to watch the full chapter summary above. If you are working through the Karp textbook, subscribing to Last Minute Lecture will supp...

Genomics, Synthetic Biology, and Evolution | Chapter 10 of Brock Biology of Microorganisms Welcome to Last Minute Lecture . This post summarizes Chapter 10 of Brock Biology of Microorganisms , which focuses on how genomics and other omics technologies revolutionize our understanding of microbes. Learn how DNA sequencing, transcriptomics, proteomics, metabolomics, and systems biology give us a complete view of microbial life — from single-cell analysis to environmental communities. 🎥 Watch the full podcast summary here: This chapter is critical for students interested in modern microbiology, bioinformatics, and synthetic biology applications in research and health. Genomics and Genome Annotation Sequencing technologies: Sanger, Illumina, Nanopore Genome assembly builds contigs and scaffolds from reads Annotation predicts gene functions using tools like BLAST Genome size in prokaryotes ≈ gene count (~1000 ORFs/Mbp) Public repositories: GenBank, GOLD Functio...